*** V42.4: New data release: March 1 2020 ***
On this page you can download a merged dataset consisting of genotypes for thousands of ancient and present-day individuals at up to 1.23 million positions in the genome (in hg19 coordinates).
All data released here:
(a) have already been published (some by our group and some by other groups - see full list of references below),
(b) have permissions appropriate for fully public data release,
(c) are typed at a set of 1,233,013 sites in the genome (or 597,573 sites for present-day individuals genotyped on the Affymetrix Human Origins array). Typing is typically pseudo-haploid for ancient samples, when coverage is too low for full genotyping.
There are two datasets:
"1240K" : Ancient and present-day individuals (from either shotgun sequencing data or in-solution target capture, with a range of coverages) at 1,233,013 sites,
"1240K+HO": Data from the above set merged with present-day individuals typed on the Human Origins array with 597,573 sites.
Each dataset consists of four files, in eigenstrat format. For details, please see:
eigensoft:
.anno: Rich meta-information for each individual.
.ind : Three columns: Individual ID, sex determination, and group label (population).
.snp : Information on each analyzed SNP position (SNP id, physical/genetic location and reference/variant alleles, where the reference allele matches hg19).
.geno: Genotypes (see note 2 below)
Version v42.4
Description |
.anno |
.ind |
.snp |
.geno |
Tarball all files |
Notes |
1240K |
link (1.7Mb) |
link (261Kb) |
link (75Mb) |
link (2.0Gb) |
link (1.6Gb) |
6522 unique individuals (3589 ancient, 2933 present-day)1 |
1240K+HO |
link (1.8Mb) |
link (384Kb) |
link (36Mb) |
link (1.5Gb) |
link (1.5Gb) |
10061 unique individuals (3589 ancient, 6472 present-day)1 |
1: includes one ancestral reference, and three present-day references: human, chimp, gorilla.
2: genotypes are in a binary form using the 'packedancestrymap' format described in in eigensoft; this may be converted to a (large) text file (i.e. 'eigenstrat' format, using the software 'convertf').
3: md5sums are available: here. These may be used to verify that files which are downloaded match this distribution, using the linux command: 'md5sum < file >'.
Please note: The unique individual identifier is given in the 'Master ID' field. Multiple representatives of the same individual are thus indicated by a duplicated master ID. Some individuals are represented more than once to reflect different versions of processing or different publications. This may happen for example, when increased coverage has been generated after an initial publication. For many analyses it may be necessary to select only one version: for example the single sample Loschbour (master ID=I0001) is represented by two instance IDs, 'Loschbour_snpAD.DG' and 'Loschbour_published.DG'. It would be incorrect to consider these as two samples from the same population. If it is not important which version is used, we suggest choosing the master ID which has the highest number of SNPs hit on autosomal targets.
We would be grateful if users of this dataset could alert us to any errors they detect and help us to fill in missing data. This could include: (1) errors or missing information for location, latitude, longitude, archaeological context, date, and group label, (2) concerns about Y chromosome or mitochondrial DNA haplogroup determinations, and (3) evidence for other problems in the data or annotations for individuals. Please write to Swapan 'Shop' Mallick and David Reich with any suggestions.
We would also be grateful if members of the community could suggest additional content that would be helpful to add to this page to make it maximally useful. Finally, please let us know if there is any ancient DNA data we should be including that we have missed.
Terminology and abbreviations used:
HG=hunter-gatherer, N=Neolithic, C=Chalcolithic/CopperAge, BA=BronzeAge, IA=IronAge
E=Early, M=Middle, L=Late, A=Antiquity
SG=samples with whole genome shotgun sequence data, randomly drawing a single read to represent each position in the genome
DG=samples shotgun sequenced with high enough coverage to call diploid genotypes, allowing for heterozygous calls
WGC=Whole Genome Capture
UDG treatments:
Various UDG protocols are available to researchers.
• UDG-minus means that no UDG treatment is used. The typical deamination profile that results is then high towards the ends of the molecules and drops off slowly as one moves 5-10 bases towards the centre of the molecule; deamination still occurs throughout the molecule.
• UDG-plus means that UDG is used. With this, molecules are cleaved where a uracil exists prior to sequencing. This means that deamination (generally) does not exist (there are some situations where UDG treatment fails, for example at methylated sites).
• UDG-half is a specialised form of UDG treatment, where uracils are not completely removed, and left at the first one or two bases. The profile of deamination is then very high at the first base, drops fast and by the third base is very low. This continues throughout the molecule, until one or two bases at the other end. This is useful in that molecules which are clearly ancient from the deamination signature may be identified (using tools such as MapDamage, or pmdtools), but the number of bases which are 'damaged' and typically have to be removed prior to analysis is low - just one or two bases, whereas with UDG-minus, typically, five or even ten bases should be removed. Given how short ancient DNA molecules can be compared with modern DNA, because of degradation, losing five or ten bases can be a considerable loss of data for some samples (in the dataset provided here, we almost always ignore data derives from sites 1-2 nucleotides from either end for UDG-half, and 5-10 nucleotides from either end for UDG-minus).
• mixed treatments, eg:UDG-plus,half: For each sample, a number of libraries may be constructed, and of course different UDG treatments can be used for different libraries. The different libraries are useful because they capture different molecules and thus increase data quality for the individual. When these libraries are combined into a single bam (or aligned dataset) for an individual sample, these can be annotated according to the separate treatments, eg: "plus,half".
Update history:
[Wed Jun 24 16:14:09 EDT 2020]: md5sums added
[Sun Mar 1 10:32:12 EDT 2020]: data release
Other versions:
V44.3: released Jan 21 2021
V37.2: released Feb 22 2019
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Ancient DNA from Chalcolithic Israel reveals the role of population mixture in cultural transformation.
Harney É, May H, Shalem D, Rohland N, Mallick S, Lazaridis I, Sarig R, Stewardson K, Nordenfelt S, Patterson N, Hershkovitz I, Reich D.
Nat Commun. 2018 Aug 20;9(1):3336. doi: 10.1038/s41467-018-05649-9. Erratum in: Nat Commun. 2018 Sep 20;9(1):3913.
[HarneyNatureCommunications2019]:
Ancient DNA from the skeletons of Roopkund Lake reveals Mediterranean migrants in India.
Harney É, Nayak A, Patterson N, Joglekar P, Mushrif-Tripathy V, Mallick S, Rohland N, Sedig J, Adamski N, Bernardos R, Broomandkhoshbacht N, Culleton BJ, Ferry M, Harper TK, Michel M, Oppenheimer J, Stewardson K, Zhang Z, Harashawaradhana, Bartwal MS, Kumar S, Diyundi SC, Roberts P, Boivin N, Kennett DJ, Thangaraj K, Reich D, Rai N.
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Early farmers from across Europe directly descended from Neolithic Aegeans.
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The Neolithic Transition in the Baltic Was Not Driven by Admixture with Early European Farmers.
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Curr Biol. 2017 Feb 20;27(4):576-582.
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A partial nuclear genome of the Jomons who lived 3000 years ago in Fukushima, Japan.
Kanzawa-Kiriyama H, Kryukov K, Jinam TA, Hosomichi K, Saso A, Suwa G, Ueda S, Yoneda M, Tajima A, Shinoda KI, Inoue I, Saitou N.
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BMC Bioinformatics. 2014 Nov 25;15:356. doi: 10.1186/s12859-014-0356-4.
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Genomic and Strontium Isotope Variation Reveal Immigration Patterns in a Viking Age Town.
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Ancient genomes suggest the eastern Pontic-Caspian steppe as the source of western Iron Age nomads.
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Ancient Fennoscandian genomes reveal origin and spread of Siberian ancestry in Europe.
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Lindo J, Achilli A, Perego UA, Archer D, Valdiosera C, Petzelt B, Mitchell J, Worl R, Dixon EJ, Fifield TE, Rasmussen M, Willerslev E, Cybulski JS, Kemp BM, DeGiorgio M, Malhi RS.
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The genetic prehistory of the Andean highlands 7000 years BP though European contact.
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Population Turnover in Remote Oceania Shortly after Initial Settlement.
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Parallel palaeogenomic transects reveal complex genetic history of early European farmers.
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Ancient West African foragers in the context of African population history.
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Ancient genomes document multiple waves of migration in Southeast Asian prehistory.
Lipson M, Cheronet O, Mallick S, Rohland N, Oxenham M, Pietrusewsky M, Pryce TO, Willis A, Matsumura H, Buckley H, Domett K, Nguyen GH, Trinh HH, Kyaw AA, Win TT, Pradier B, Broomandkhoshbacht N, Candilio F, Changmai P, Fernandes D, Ferry M, Gamarra B, Harney E, Kampuansai J, Kutanan W, Michel M, Novak M, Oppenheimer J, Sirak K, Stewardson K, Zhang Z, Flegontov P, Pinhasi R, Reich D.
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Ancient Ethiopian genome reveals extensive Eurasian admixture throughout the African continent.
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Two ancient human genomes reveal Polynesian ancestry among the indigenous Botocudos of Brazil.
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The Simons Genome Diversity Project: 300 genomes from 142 diverse populations.
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The genomic ancestry of the Scandinavian Battle Axe Culture people and their relation to the broader Corded Ware horizon.
Malmström H, Günther T, Svensson EM, Juras A, Fraser M, Munters AR, Pospieszny L , Tõrv M, Lindström J, Götherström A, Storå J, Jakobsson M. Proc Biol Sci. 2019 Oct 9;286(1912):20191528. doi: 10.1098/rspb.2019.1528. Epub 2019 Oct 9. PMID: 31594508.
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Genomic signals of migration and continuity in Britain before the Anglo-Saxons.
Martiniano R, Caffell A, Holst M, Hunter-Mann K, Montgomery J, Müldner G, McLaughlin RL, Teasdale MD, van Rheenen W, Veldink JH, van den Berg LH, Hardiman O, Carroll M, Roskams S, Oxley J, Morgan C, Thomas MG, Barnes I, McDonnell C, Collins MJ, Bradley DG.
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The population genomics of archaeological transition in west Iberia: Investigation of ancient substructure using imputation and haplotype-based methods.
Martiniano R, Cassidy LM, Ó'Maoldúin R, McLaughlin R, Silva NM, Manco L, Fidalgo D, Pereira T, Coelho MJ, Serra M, Burger J, Parreira R, Moran E, Valera AC, Porfirio E, Boaventura R, Silva AM, Bradley DG.
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Genome-wide patterns of selection in 230 ancient Eurasians.
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Nature. 2015 Dec 24;528(7583):499-503. doi: 10.1038/nature16152. Epub 2015 Nov 23.
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The genomic history of southeastern Europe.
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POPULATION GENETICS. Genomic evidence for the Pleistocene and recent population history of Native Americans.
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The genome of a Late Pleistocene human from a Clovis burial site in western Montana.
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The ancestry and affiliations of Kennewick Man.
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Ancient human parallel lineages within North America contributed to a coastal expansion.
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Megalithic tombs in western and northern Neolithic Europe were linked to a kindred society.
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[ScheunemannNatureCommunications2017]:
Ancient Egyptian mummy genomes suggest an increase of Sub-Saharan African ancestry in post-Roman periods.
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Nat Commun. 2017 May 30;8:15694. doi: 10.1038/ncomms15694.
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Iron Age and Anglo-Saxon genomes from East England reveal British migration history.
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Nat Commun. 2016 Jan 19;7:10408. doi: 10.1038/ncomms10408.
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Southern African ancient genomes estimate modern human divergence to 350,000 to 260,000 years ago.
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The population history of northeastern Siberia since the Pleistocene.
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Ancient genomes show social and reproductive behavior of early Upper Paleolithic foragers.
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Ancient genomes show social and reproductive behavior of early Upper Paleolithic foragers.
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Reconstructing Prehistoric African Population Structure.
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Genetic evidence for two founding populations of the Americas.
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[SkoglundNature2016]:
Genomic insights into the peopling of the Southwest Pacific.
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Ancestry and demography and descendants of Iron Age nomads of the Eurasian Steppe.
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Four millennia of Iberian biomolecular prehistory illustrate the impact of prehistoric migrations at the far end of Eurasia.
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Pleistocene North African genomes link Near Eastern and sub-Saharan African human populations.
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A Late Bronze Age II clay coffin from Tel Shaddudin the Central Jezreel Valley, Israel: context andhistorical implications.
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Population genomic analysis of elongated skulls reveals extensive female-biased immigration in Early Medieval Bavaria.
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Testing support for the northern and southern dispersal routes out of Africa: an analysis of Levantine and southern Arabian populations.
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Ancient human genome-wide data from a 3000-year interval in the Caucasus corresponds with eco-geographic regions.
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[YangCurrentBiology2017]:
40,000-Year-Old Individual from Asia Provides Insight into Early Population Structure in Eurasia.
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[ZallouaScientificReports2018]:
Ancient DNA of Phoenician remains indicates discontinuity in the settlement history of Ibiza.
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Update history:
Sat Feb 29 15:44:38 EST 2020: V42.4 update
Tue Apr 2 18:07:09 EDT 2019: edits
Mon Apr 1 22:15:31 EDT 2019: website integration edits
Thu Mar 28 23:33:13 EDT 2019: V37.2 minor edits.
Tue Mar 26 11:59:31 EDT 2019: V37.2 minor edits.
Mon Mar 25 16:26:10 EDT 2019: V37.2 tidy .anno.
Fri Feb 22 12:25:37 EST 2019: V37.2 new release.